Xenbase Image ID: 1016

Fig. 7. Vent2 mediates β-catenin function. (A) Xenopus embryos were injected with the indicated hhex:luciferase constructs with or without Vent2 RNA (500 pg) in D1 anterior or D4 posterior cells at the 32-cell stage. The bar chart shows the normalized relative luciferase activity at gastrula stage, indicating that Vent2 represses the hhex promoter. (B-D) In situ hybridization of bisected stage-18 embryos with the probes indicated. (E) Injection of Gsk3β RNA (500 pg) in the posterior endoderm repressed vent2 expression. (F) Embryos were injected at the 32-cell stage with Vent2 RNA in anterior D1 cells or in posterior D4 cells with either Gsk3β or Gsk3β plus Vent2, followed by in situ hybridization at stage 18 with hhex, and stage 35 with for1 or pdx1 probes. (G) These data suggest a molecular pathway in which Wnt/β-catenin signaling promotes vent2 expression and Vent2 represses hhex transcription. Image published in: McLin VA et al. (2007) Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Throughout NF stage 18 archenteron nr1h5.L for1 X. laevis Throughout NF stage 35 and 36 liver pdx1.S ipf1, pdx-1, STF-1, XlHbox-8, XlHbox8 X. laevis Throughout NF stage 35 and 36 exocrine pancreas duodenum hhex.L hex, tHex, XHex X. laevis Throughout NF stage 18 archenteron ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 18 mesoderm posterior endoderm wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 18 lateral plate mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + gsk3b + NF18 (in situ hybridization) Fig.7.E Expression Phenotype decreased amount ventx2.2.L expression in posterior endoderm Xla Wt + gsk3b + NF18 (in situ hybridization) Fig.7.F Expression Phenotype mislocalised hhex.L expression in endoderm Xla Wt + gsk3b + NF35/36 (in situ hybridization) Fig.7.F Expression Phenotype mislocalised nr1h5.L expression in endoderm Xla Wt + ventx2.2 + NF18 (in situ hybridization) Fig.7.F Expression Phenotype decreased amount hhex.L expression in anterior endoderm Xla Wt + ventx2.2 + NF35/36 (in situ hybridization) Fig.7.F Expression Phenotype decreased amount nr1h5.L expression in liver decreased amount pdx1.L expression in dorsal pancreatic bud decreased amount pdx1.L expression in ventral pancreatic bud Larger Image Printer Friendly View

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