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Figure 7. Sequence Determinants Conferring EGF-CFC DependenceSynthetic mRNAs (200 pg) encoding ActivinβB with single or double region substitutions from Sqt were injected into wild-type and MZoep embryos. gsc and ntl mRNA expression is shown at shield stage; animal pole views are dorsal to the right. Schematic is not drawn to scale. HA indicates a hemagglutinin epitope tag. Note that ActSqt[loopβ8] containing the loop-β8 region of Sqt is inactive in both wild-type and MZoep embryos. In ActSqt[Finger1-loopβ8], the additional substitution of Sqt Finger 1 region relieves the inhibitory presence of the Sqt loop-β8 region. Similar to Sqt, ActSqt[Finger1-loopβ8] can induce ectopic gsc and ntl in wild-type, but not in MZoep embryos. Western blot analysis indicates that these chimeric constructs produce stable ligands (data not shown). Image published in: Cheng SK et al. (2004) Copyright: ©2004 Cheng et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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