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Figure 8. EGF-CFC Coreceptor Depen-dence Determines Susceptibility to Antagonism by Lefty(A–L) Embryos were injected with 75 pg of SqtActβB[loopβ8] mRNA (A–C), 75 pg of SqtActβA[loopβ8] mRNA (D–F), 200 pg of Vg1ActβB[loopβ8] mRNA (G–I), or 200 pg ActSqt[Finger1-loopβ8] mRNA (J–L). Embryos were further double-injected with either 500 pg of LacZ mRNA (A, D, G, and J), 100 pg of lefty1 and 400 pg LacZ mRNAs (B, E, H, and K), or 500 pg of lefty1 mRNA (C, F, I, and L). gsc mRNA expression in wild-type zebrafish embryos is shown at shield stage, animal pole view. Note that both levels of Lefty1 cannot inhibit the ectopic gsc expression induced by SqtActβB[loopβ8] (B and C), SqtActβA[loopβ8] (E and F), and Vg1ActβB[loopβ8] (H and I). In contrast, Lefty1 can inhibit ActSqt[Finger1-loopβ8] (K and L).(M) Wild-type embryos were injected with 75 pg of either SqtActβB[loopβ8], SqtActβA[loopβ8], Vg1ActβB[loopβ8], or 200 pg of ActSqt[Finger1-loopβ8] mRNA. Embryos were further double-injected with 500 pg of LacZ mRNA, 100 pg of lefty1, and 400 pg of LacZ mRNAs, or 500 pg of lefty1 mRNA. Smad2 pathway activation was measured by an Activin response element luciferase reporter, A3-luc. Values are folds over wild-type control injected with 500 pg of LacZ mRNA and A3-luc reporter. An asterisk indicates a significant difference from the level of activation with ligand and LacZ expression alone (Student's t-test, p < 0.05). Image published in: Cheng SK et al. (2004) Copyright: ©2004 Cheng et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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