XB-IMG-116381
Xenbase Image ID: 116381
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Figure 6. Anterior Neural Induction in Keller Explants Requires Chd(A) Proposed vertical and planar signals in neural induction (following Ruiz i Altaba 1993).(B) Diagram of Keller explant preparation and subsequent elongation of the endomesoderm by convergent extension (Keller 1991).(C) The neural and mesodermal regions of Keller explants contain descendants of BCNE cells (in blue) marked by blastomere injection at the 64-cell stage.(D) Expression of Otx2 and Krox20 in Keller explants (n = 7).(E) Injection of 17 ng Chd-MO completely blocked Otx2 and Krox20 expression in neural regions, while expression of Otx2 in anterior endoderm was not affected (n = 10).(F) The differentiated neuron marker N-tubulin is expressed in Keller explants (n = 8).(G) Partial inhibition of N-tubulin by injection of Chd-MO (n = 7).(H and I) Summary of the effects of Chd-MO in Keller explants. Abbreviations: SC, spinal cord; CG, cement gland; Epi, epidermis.(J) RT-PCR analyses of the effect of Chd-MO in Keller explants; samples injected with (plus) or without (minus) Chd-MO are indicated. Lane 1, whole embryos; lanes 2–7, Keller sandwiches. Note that expression of the neural markers NCAM and N-tubulin in Keller sandwiches was abolished by co-injection with 200 pg of dnFGF receptor 4a (dnFGF4a) mRNA and 17 ng of Chd-MO (lane 5). Injection with 600 pg of CerS mRNA, which eliminates mesoderm but not BCNE formation, does not affect neural induction in this assay (lane 6). Image published in: Kuroda H et al. (2004) Copyright: © 2004 Kuroda et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |