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Figure 2. The BCNE Center Contributes to Forebrain and Midline Structures(A) Method used for lineage tracing of the BCNE center with biotin-dextran amine (BDA) labeled grafts.(B) Sagittal section of a recently grafted BCNE at stage 9.(C) Chd mRNA expression at stage 9.(D) BCNE descendants at stage 10.5.(E) Chd mRNA expression at stage 10.5.(F) BCNE center descendants at stage 11.(G) Dorsal view of BCNE descendants at neural plate stage 14.(H) Double staining of transplanted BCNE region with nuclear lacZ mRNA and epidermal ectoderm of the host with epidermal cytokeratin (epi) probe in light red at stage 14.(I) Transverse section at the level of the trunk at stage 16. Abbreviations: fp, floor plate; no, notochord.(J–L) Transverse sections at stage 40. Abbreviations: fp, floor plate; hb, hindbrain; he, heart; le, lens; mb, midbrain; no, notochord; ov, otic vesicle; re, retina.(M) Dorsal view of 6-d embryo transplanted with a BCNE graft from CMV-GFP transgenic embryos. Abbreviations: br, brain; fp, floor plate; on, optic nerve; op, olfactory placode.(N) Side view at 4 d showing labeled retina and brain. Abbreviation: br, brain. Image published in: Kuroda H et al. (2004) Copyright: © 2004 Kuroda et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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