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Figure 12. MagT1 mRNA expression is responsive to magnesium. Where indicated MDCT cells were cultured in normal (1.0 mM) or low (<0.01 mM) magnesium media for 16 h. Kidney cortical tissue was harvested from mice on normal (0.05% by weight) or low magnesium (<0.01%) diets for 5 days. MagT1 and murine β-actin RNA was measured with Real-Time RT PCR (AB7000TM, Applied Biosystems) using SYBR GreenTM fluorescence. Data is from 10-12 PCRs performed on five separate cultures or animals in each group maintained on low and normal magnesium. Image published in: Goytain A and Quamme GA (2005) Copyright © 2005 Goytain and Quamme; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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