Xenbase Image ID: 116535

Figure 2. The gata2 promoter directs reporter gene activity that is induced by BMP4 and dependent on Smad signaling. (A) The luciferase reporter was injected at the four-cell stage into blastomeres that contribute preferentially to ventral or dorsal regions of the embryo, as indicated. The reporter is more active in the ventral-posterior derivatives, but this is abrogated by co-injection of RNA encoding the inhibitory Smad6 (the error bar for this sample is present, but it is so small that it is not evident). The results indicate that the reporter responds as predicted to the endogenous Smad-dependent BMP signaling pathway. (B) The gata2 promoter is induced ∼5-fold by co-injection of RNA encoding BMP4. This activation is also blocked by co-expression of Smad6. Image published in: Oren T et al. (2005) Image downloaded from an Open Access article in PubMed Central. © The Author 2005. Published by Oxford University Press. All rights reserved Larger Image Printer Friendly View

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