XB-IMG-116539
Xenbase Image ID: 116539
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Figure 6. The octamer site of BRE1 binds Oct-1 in Xenopus nuclear lysates. (A) Gel mobility shift assays were performed using a labeled probe containing the BRE1 sequences including the putative octamer binding site. Lane 0 represents probe alone and the position of the free probe is indicated (P). In lanes marked with a ‘+’, a specific complex forms using nuclear extracts derived from stage 13 embryos, as indicated by the arrow in the second lane. The complex is competed specifically by the addition in the reaction of excess (10×, 25× or 100×) unlabeled probe DNA (self) or DNA containing the octamer consensus (octamer), but not by DNA containing the same mutation of the octamer that blocks the induction by BMP4 (mutant). (B) Similar gel mobility-shift assays were performed. In this case, the lanes include probe alone (0), nuclear extract (1), nuclear extract and antibody to Xenopus Oct-1 (2) or a control isotype-matched antibody (3). The free probe is indicated (P) as are the positions of a non-specific complex (NS), the specific complex (SP) and the complex that is super-shifted by addition of the Oct-1 antibody (SS). Image published in: Oren T et al. (2005) Image downloaded from an Open Access article in PubMed Central. © The Author 2005. Published by Oxford University Press. All rights reserved Larger Image Printer Friendly View |