Xenbase Image ID: 116825

Figure 1. The Xenopus Xvsx1 Homeobox Gene Supports Bipolar Cell Fate(A–C) Sections of st. 42-lipofected retinas. GFP (green) traces lipofection. (A) Example of control lipofection. (B and C) Example of Xvsx1 lipofection. (C) Immunostaining (red fluorescence) with amacrine antibodies panel (anti-5-HT, anti-GABA, anti-tyrosine hydroxilase), labeling the main classes of amacrine cells at this developmental time [47,48] ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Xvsx1 lipofection (B) increases the proportion of INL cells and decreases the proportion of ONL cells compared to control (A). The majority of the Xvsx1-lipofected cells in the INL are not stained either by amacrine markers (C), or by the horizontal marker prox1 (not shown).(D) Statistical analysis showing the proportion of lipofected cell types. Cell types were identified as described [14]. Error bars indicate standard error of the mean. Xvsx1 misexpression increases the proportion of bipolar cells (from 33% of control to 55%, student's t-test, p = 0.000043), mainly at the expense of photoreceptors (from 29% to 14%, student's t-test, p = 0.000011). Image published in: Decembrini S et al. (2006) Copyright: © 2006 Decembrini et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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