Xenbase Image ID: 116827

Figure 3. The 3′ UTRs of Xotx5b, Xvsx1, and Xotx2 mRNA Direct Cell Type–Specific Inhibition of Translation(A) Detection of sensor mRNAs (Fast Red), sensor GFP protein (green immunodetection), or co-detection of both mRNA and protein (merge, yellow), in cells of mature retinas (st. 42) lipofected with GFP control vector, or UTR-carrying sensor vectors (see Materials and Methods). Unlike control GFP, sensor GFP translation is detectable (arrowheads) mainly in photoreceptors (Xotx5b sensor) or bipolar cells (Xvsx1 and Xotx2 sensors). ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer.(B) Bars show the proportion of sensor-translating/sensor-transcribing cell types. Number of cells is indicated by n. Error bars represent standard error of the mean. Single asterisk indicates p ≤ 0.05; double asterisk indicates p ≤ 0.01 (student's t-test). Image published in: Decembrini S et al. (2006) Copyright: © 2006 Decembrini et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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