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Figure 5. Blocking Cell Cycle by HUA Inhibits the Sequential Translation of Xotx5b, Xvsx1, and Xotx2 mRNAsIn situ hybridization (mRNA: Fast Red detection) and antibody immunodetection (protein: green fluorescence) on 10-μm thick serial sections of st. 42 retinas. Embryos were grown in medium containing HUA (hydroxyurea, 150 μM and aphidicolin, 20 μM) from the stage indicated to st. 42. The examples show typical results obtained in three different experiments (n > 15 embryos/treatment in each experiment). Image published in: Decembrini S et al. (2006) Copyright: © 2006 Decembrini et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |