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Figure 4. MT Stabilizing Activities of EB1 and XMAP215 in Interphase and Metaphase Xenopus Egg Extracts(A) Average size of asters in response to depletion of XMAP215 (ΔXMAP215) from the extract and the addition of recombinant EB1 to the ΔXMAP215 extract. Error bars represent the S.D. (n > 30). Upper panel: immunoblot (IB) of MAPs after indicated treatments.(B) Average size of asters in response to depletion of EB1 (ΔEB1) from the extract and the addition of recombinant XMAP215 to the ΔEB1 extract. Error bars represent the S.D. (n > 30). Upper panel shows the immunoblot (IB) of MAPs after the indicated treatments.(C) Still images of MT asters after the indicated treatments are shown in the upper panel. Table summarizing the dynamic parameters of MT growth in response to EB1 depletion (ΔEB1) and add back of recombinant EB1 into ΔEB1 interphase extracts is shown in the lower panel.

Image published in: Niethammer P et al. (2007)

Copyright: © 2007 Niethammer et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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