Xenbase Image ID: 116918

Figure 6. The MT Destabilizing Activity of XKCM1 Is Inhibited by MAPs in Interphase Egg Extracts(A) Still images and table summarizing the dynamic parameters of MT growth in response to XKCM1 depletion (ΔXKCM1) and add back of the recombinant protein.(B) Aster size after stepwise addition of recombinant XKCM1 into undepleted interphase and metaphase extracts. Error bars represent the S.D. (n > 30). Inset shows the Western blot of extracts containing increasing amounts of recombinant XKCM1 (XKCM1rec = amount of XKCM1 added in nM; and IB: XKCM1 = immunoblot with anti-XKCM1 antibody).(C) Aster size after stepwise addition of recombinant XKCM1 into control (IgG) depleted (indigo line), XMAP215 depleted plus 40 nM XMAP215 (red line), APC depleted (pink line), EB1 depleted (dark yellow line), and APC and EB1 depleted (cyan line) interphase extracts. Error bars represent the S.D. (n > 30).(D) Western blot of extracts containing increasing amounts of recombinant XKCM1 (XKCM1rec = amount of XKCM1 added in nM; and IB: XKCM1 = immunoblot with anti-XKCM1 antibody) that were depleted of specified MAPs. Image published in: Niethammer P et al. (2007) Copyright: © 2007 Niethammer et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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