Xenbase Image ID: 117102

Figure 1. Gsc knockdown in Xenopus embryos causes loss of head structures and affects patterning of the AP and DV axes. (A) Gsc marks Spemann organizer endomesoderm at early gastrula. (B) Gsc MO targets both pseudoalleles of the X. laevis Gsc gene. (C–I) Gsc MO injection (136 ng total) causes loss of head structures, marked by Otx2 (forebrain), Six3 and Rx2a (forebrain and eyes), and En2 (midbrain/hindbrain border) (n=106; Supplementary Table I). Expression of the ventral marker Szl is reduced anteriorly and expanded posteriorly in the ventral blood island. (E) Co-injection of mGsc mRNA (200 pg total, radial injection) rescues the Gsc MO phenotype (n=78). (H, I) Knockdown of Gsc reduces head size and affects patterning of the posterior somites, including loss of MyoD expression at the tip of the tail (arrows). (J, K) Moderately affected embryos survive until tadpole stage and have cyclopic eyes (indicating holoprosencephaly) and no mouth opening. Image published in: Sander V et al. (2007) Copyright © 2007, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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