Xenbase Image ID: 117103

Figure 2. The dorsalizing effects of mGsc mRNA injection require Chd. (A–E) Gsc MO reduces Chd expression at gastrula 2.5-fold, whereas overexpression of mGsc mRNA greatly expands Chd expression. (F–H) Injection of 50 pg mGsc mRNA into one ventral blastomere at the four-cell stage leads to a range of dorsalized phenotypes, of which 50% develop secondary axes (38% partial; 12% complete with eyes, notochords, and cement glands). (I, J) Co-injection of Chd MO (34 ng) prevents second axis induction and dorsalization by mGsc mRNA in 97% of the embryos. (K, L) mGsc mRNA microinjection (200 pg total) induces Chd expression in UV-ventralized embryos at gastrula. (M–O) The rescue of head (Otx2) and pan-neural marker (Sox2) in UV embryos by mGsc overexpression (n=54) has a complete requirement for Chd (co-injection of 136 ng Chd MO; n=59). Image published in: Sander V et al. (2007) Copyright © 2007, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

Return to previous page