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Figure 4. Double depletion of Vent1 and Vent2 causes severe dorsalization of the embryo. (A–D) Injection of either Vent1 or Vent2 MO expands the neural plate at neurula stage (insets), but only the combination of both MOs strongly dorsalizes tailbud stage embryos, with shortened body axes and large heads and cement glands (n=122; Supplementary Table I). (E, F) Vent1/2 depletion leads to transcriptional upregulation of Gsc (hemisections at stage 10; n=15) and Chd (insets in panels E and F; whole embryos, vegetal view; n=18). (G, H) Loss of Gsc increases Vent1 and Vent2 expression (hemisections at stage 10; n=21 and 15) (I) Quantitative RT–PCR analyses showing 3- to 4-fold upregulation of the organizer genes Gsc, Chd, and Admp in animal caps at gastrula stage after Vent1/2 depletion. Image published in: Sander V et al. (2007) Copyright © 2007, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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