Xenbase Image ID: 117109

Fig. 1. Motor attachment, experimental setup and bead traces. a–d, f Possible Eg5-motor attachments to beads (silica, 0.5 μm diameter) via the genetically encoded N-terminal His-tag of Eg5. a All four motor domains are bound, preventing motility. b One motor domain is unbound, likely allowing only non-processive motility. c Two motor domains are free, one at each end, likely allowing only non-processive motility. d One motor domain is bound, leaving a dimeric motor end free to interact with the microtubule. e Traces of bead motility generated by individual Eg5 (green) and Kinesin-1 (grey) motors. The averaged (15-point) and median-filtered (0.3 s (Eg5) and 0.05 s (Kinesin-1) sliding windows; rank 10) signal is overlaid in red over both traces. The trap stiffnesses were 0.03 pN/nm (Kinesin-1) and 0.013 pN/nm (Eg5). f One dimer is bound, one dimer is free; sketch of a silica-sphere with motor held in the laser-trap, such that it interacts with a surface-attached microtubule track Image published in: Korneev MJ et al. (2007) Image downloaded from an Open Access article in PubMed Central. © EBSA 2007 Larger Image Printer Friendly View

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