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Figure 3. Representative RT-PCR Results of Similar MT1-MMP and TIMP-2 Levels of RNA During Natural and T3 Induced Metamorphosis.PCR products were agarose gel fractionated, stained, and photographed. MT1-MMP and TIMP-2 transcription patterns mimic each other at all stages and conditions. In the intestine, MT1-MMP and TIMP-2 transcripts are poorly detected at stage 56 prior to metamorphosis. Levels of both increase during stages 58, 61 and 63 and then drop to pre-metamorphic levels at stage 65. Conversely, in the tail, transcripts are detectable at stage 56, rise to stage 61, and then begin to drop at stage 63. T3 treatment induces a drop in levels after one day, but an increase after three days for both genes. EF1α RNA levels are shown for all comparable stages and treatments. Image published in: Walsh LA et al. (2007) Walsh et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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