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Figure 5. Determination of the nucleotides opposite the AP site in the final replication products (after 75 min of incubation in NPE). (A) Restriction digestion of the gel-purified supercoiled final replication products (detected by 32P) and the control pET28a DNA (detected by SYBR Gold). R: relaxed; L: linear; S: supercoiled. (B) Transformation efficiency of pET28a (kanR; expressed in percentages of the number of transformants of the uncut DNA) and AP DNA (ampR; expressed in absolute colony numbers). (C) The nucleotides found at the position opposite the AP lesion in the plasmids isolated from the transformants of the DpnI and ClaI-digested Δ:C and Δ:G replication products. (D) The average ratios and absolute deviations of each nucleotide inserted at the position opposite the AP lesion for the Δ:C and Δ:G replication products. The data were from two independent experiments for each substrate. The right-most column listed the expected ratios if the AP lesion was replicated by random insertion of the 4 nt. Image published in: Liao S et al. (2007) © 2007 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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