Xenbase Image ID: 117495

Figure 5. Wnts, but not BVg1 or noggin, are able to modulate ectopic β-catenin–myc accumulation both before and after MBT, as assayed by anti–c-myc immunostaining. Uninjected controls do not stain for c-myc when assayed before (A) or after (B) MBT. Embryos expressing β-catenin–myc and prolactin display a dorsal accumulation of ectopic β-catenin–myc both before (C) and after (D) MBT, while the ventral side shows negligible staining. (E) Xwnt-8 RNA expands the domain of accumulation of β-catenin–myc when assayed before MBT (shown, 100 pg Xwnt-8 RNA was injected for the embryo on the left, and 3 ng RNA for the embryo on the right) or after MBT (see Table I). (F) Neither BVg1 (pre-MBT, shown; and post-MBT, Table I) nor noggin RNA (see Table I) are able to elevate β-catenin–myc accumulation on the ventral side, while β-catenin–myc accumulates on the dorsal side similarly to control embryos. (G) Xwnt-5A RNA does not alter the pattern of β-catenin–myc accumulation during the early cleavage stages, but causes β-catenin–myc to accumulate around the entire marginal zone after MBT (H). In these experiments, 1 ng of β-catenin RNA and 3 ng of prolactin, Xwnt-8 (100 pg in left embryo of panel E), Xwnt-5A, BVg1, or noggin RNA were injected into the marginal zone of each blastomere of four cell stage embryos, followed by anti–c-myc immunostaining before and after MBT. Arrowheads denote dorsal (single arrowhead) or dorsal and ventral (double arrowhead) staining. Image published in: Larabell CA et al. (1997) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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