Xenbase Image ID: 118093

Figure 6. GFP-CT44del25, but not endogenous rhodopsin, accumulates abnormally in the Golgi, RIS plasma membrane, and synapse. EM micrographs of a rod cell expressing GFP-CT44del25. The cell was labeled either with anti-GFP antibody (A) or antirhodopsin antibody, 11D5 (B), followed by a gold-conjugated secondary antibody. The boxed regions are magnified to show detail. Arrows point to GFP (A 1) and rhodopsin (B 1) labeling present in the Golgi. Arrowheads indicate the presence of GFP-CT44del25 (A 2), but not rhodopsin (B 2), on the RIS plasma membrane. Likewise, GFP-CT44del25 (A 3) is abundant in the synaptic terminal; rhodopsin (B 3) is not. Therefore, delocalization of the fusion protein does not result in delocalization of endogenous rhodopsin. os, outer segment; is, inner segment; n, nucleus; m, mitochondria; G, Golgi; and S, synaptic terminal. Bar, 2.5 μm. Image published in: Tam BM et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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