Xenbase Image ID: 118093
Figure 6. GFP-CT44del25, but not endogenous rhodopsin, accumulates abnormally in the Golgi, RIS plasma membrane, and synapse. EM micrographs of a rod cell expressing GFP-CT44del25. The cell was labeled either with anti-GFP antibody (A) or antirhodopsin antibody, 11D5 (B), followed by a gold-conjugated secondary antibody. The boxed regions are magnified to show detail. Arrows point to GFP (A 1) and rhodopsin (B 1) labeling present in the Golgi. Arrowheads indicate the presence of GFP-CT44del25 (A 2), but not rhodopsin (B 2), on the RIS plasma membrane. Likewise, GFP-CT44del25 (A 3) is abundant in the synaptic terminal; rhodopsin (B 3) is not. Therefore, delocalization of the fusion protein does not result in delocalization of endogenous rhodopsin. os, outer segment; is, inner segment; n, nucleus; m, mitochondria; G, Golgi; and S, synaptic terminal. Bar, 2.5 μm.
Image published in: Tam BM et al. (2000)
Image downloaded from an Open Access article in PubMed Central. © 2000 The Rockefeller University Press
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