Xenbase Image ID: 118096
Figure 5. Rhodopsin's outer segment targeting/retention signal resides within its distal most eight amino acids. Confocal micrographs of transgenic retinas expressing (A) GFP-AAR; (B) GFP-AAR(CC); (C) GFP-AAR(CC)rho8; and (D) GFP-AAR(CC)rho6. GFP-AAR distributed mainly to RIS membranes, especially the synaptic terminal, but was also partially soluble. GFP-AAR(CC) displayed greater membrane association than its single cysteine counterpart and localized to both ROS and RIS membranes. GFP-AAR(CC)rho8 localized exclusively to the ROS while GFP-AAR(CC)rho6 localized to both ROS and RIS membranes. The last eight amino acids of rhodopsin are therefore sufficient to direct ROS localization. GFP (green) and Hoescht 33342 (blue). os, outer segment; is, inner segment; n, nucleus; and s, synaptic terminal. Bar, 5 μm.
Image published in: Tam BM et al. (2000)
Image downloaded from an Open Access article in PubMed Central. © 2000 The Rockefeller University Press
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