Xenbase Image ID: 118122

Figure 6. The WH1 domain is not required for the ability of PI(4,5)P2 to activate N-WASP. (A) A 10% SDS–polyacrylamide gel showing purified protein preparations of GST-tagged wild-type N-WASP (GST-N-WASP) or a GST-tagged truncation mutant of N-WASP lacking the WH1 domain (GST-(ΔWH1) N-WASP). (B) Actin polymerization (1.3 μM actin, 30 nM Arp2/3 complex) in the presence of the indicated combinations of GST-N-WASP (50 nM), GST-(ΔWH1)N-WASP (50 nM), Cdc42-GTPγS (100 nM), or PI(4,5)P2-containing vesicles (1 μM). Image published in: Rohatgi R et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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