Xenbase Image ID: 118125

Figure 9. N-WASP is required for PI(4,5)P2-stimulated actin polymerization in Xenopus HSS. (A) Affinity-purified α-N-WASP antibody or antibody buffer (mock) was used to immunodeplete N-WASP from Xenopus HSS, and the material in the supernatant was analyzed for the presence of N-WASP by immunoblotting using the same α-N-WASP antibody. (B) Comparison of actin assembly stimulated by 100 μM PI(4,5)P2-containing vesicles in mock-depleted HSS, α-N-WASP–depleted HSS, and in depleted HSS reconstituted with 50 nM recombinant N-WASP. The add back of recombinant N-WASP to α-N-WASP–depleted extracts and the addition of PI(4,5)P2-containing vesicles was performed at the time points indicated by the arrows. (C and D) The abilities of GST-N-WASP, GST-(ΔWH1)N-WASP, or GST-(ΔB)N-WASP (100 nM each) to restore PI(4,5)P2-stimulated actin polymerization to HSS depleted of endogenous N-WASP. Image published in: Rohatgi R et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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