Xenbase Image ID: 118309

Figure 4. NO3− and SCN− current by slc26a3. Xenopus oocytes expressing slc26a3 and bathed in HEPES-buffered media (A–C) were incubated in media in which Clo− was replaced with NO3− (A and B) or SCN− (C) while measuring the I-V relationship (A) or the current (B and C) at a holding membrane potential of −30 mV and sampling every 10 s by stepping to −100 and 60 mV for 50 ms. In A, the oocyte was incubated in Cl−-containing media (squares) or NO3−-containing media for 1 (circles), 3 (triangles), 5 (diamonds), or 10 min (stars). B and C show the current measured in control (circles) and slc26a3 (squares)-expressing oocytes. The models in B and C show the possible modes of transport at the beginning and end of the incubation period with NO3− and SCN−. (D) The I-V relationship in an HEK293 cell transfected with GFP (squares) or with GFP and slc26a3 and dialyzed with NO3i− and incubated in Na+-free medium in which the major anion was NO3− (squares and triangles), gluconate (inverted triangles), or Cl− (circles). The traces in A–D are from single experiments, and the means and number of experiments are given in the text. Image published in: Shcheynikov N et al. (2006) Copyright © 2006, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page