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Figure 6. Stoichiometry of Cl−/HCO3− exchange by slc26a6. (A) Xenopus oocyte expressing slc26a6 and bathed in HCO3−-buffered media was incubated in Cl−-free and Cl−-containing medium as indicated. The rates of HCO3− (heavy black trace) and Cl− (heavy gray trace) transport initiated by the removal of Clo− were used to calculate the Cl−/HCO3− transport stoichiometry of slc26a6, and the results of multiple experiments are given in Table 1. The light gray trace shows the change in membrane potential. (B) The current was measured in an oocyte expressing slc26a6 and bathed in HCO3−-buffered media. Where indicated, the membrane potential was clamped at 40 mV, and the effect of Cl− removal and readdition on the current was measured. (C) The HEK293 cell expressing slc26a6 was dialyzed with Na+-free pipette solution containing 150 mM Cli− and bathed in Na+-free solutions containing 150 mM Cl− (squares), gluconate (circles), Cl− and 10 mM oxalate (triangles), or gluconate and oxalate (inverted triangles), and the I-V relationship was determined between −80 and 60 mV. The number of experiments and means are given in the text. Image published in: Shcheynikov N et al. (2006) Copyright © 2006, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |