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Figure 8. Coupling of Cl− and HCO3− transport by slc26a6. (A) An oocyte expressing slc26a6 was incubated in Cl−-free medium while bathed in HEPES-buffered and HCO3−-buffered media. Red trace, pHi; green trace, Cli−; black trace, membrane potential. This experiment is representative of three others with similar results. (B) An oocyte expressing slc26a6 and bathed in HCO3−-buffered media was incubated in Cl−-free medium, and, shortly after the removal of Clo−, 25 μM DIDS was added to the perfusate, which halted the Cl− (green trace) and HCO3− (red trace) fluxes and reversed the hyperpolarization (black trace). (C) Summary of the changes in pHi (red bars), Cli− (green bars), and membrane potential (MP; black bars) recorded in four experiments in which oocytes expressing slc26a6 bathed in HCO3−-buffered media were incubated with either 1 or 5 μM DIDS before the incubation in Cl−-free media that contained the respective concentrations of DIDS. The effect of preincubation with 25 μM DIDS, which completely inhibited the fluxes and the associated change in membrane potential, was taken as 100% to calculate the percent inhibition by 1 and 5 μM DIDS. Error bars represent SEM. Image published in: Shcheynikov N et al. (2006) Copyright © 2006, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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