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Figure 1. Phosphorylation of AQP2 in oocytes. Oocytes, injected with wt-AQP2 or AQP2-S256A cRNA, were labeled with [32P]orthophosphate for 2 d. Subsequently, oocytes were not stimulated (ctrl, AQP2-S256A), or were incubated with 5 × 10−4 M 8-Br-cAMP or 10−4 M H89 for the indicated time periods. Total membranes were isolated and divided into two portions. One portion was solubilized, AQP2 was immunoprecipitated and subjected to PAGE, the gel was dried, and labeled AQP2 (p-AQP2) was visualized using autoradiography. The other portion was directly immunoblotted for AQP2 (Total AQP2). Image published in: Kamsteeg EJ et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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