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Figure 6. The relation between the expressed amounts and conferred water permeabilities of AQP2-S256D-F. Of oocytes, injected with 0.1, 0.2, 0.3, or 0.4 ng of AQP2-S256D-F cRNA, the water permeability (Pf ± SEM in μm/s) was measured. Total membranes were isolated and immunoblotted for AQP2 (AQP2-S256D-F; insert). The signals were scanned and the amounts of expressed AQP2-S256D-F (AQP2 amount in arbitrary units) were semiquantified as described in the legend to Fig. 5. The linear relation between the amount of AQP2-S256D-F protein and the conferred water permeability was: Pf = (3.97 × amount of AQP2 protein expected in membrane) − 9. 7. For expression levels lower than obtained for 0.1 ng AQP2-S256D-F cRNA, the relation was extrapolated to the Pf level of control oocytes (8 ± 4 μm/s, dotted line). Image published in: Kamsteeg EJ et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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