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Figure 2. SA1 and SA2 are subunits of two distinct human 14S cohesin complexes. (A) Low-speed supernatant of extracts from logarithmically growing HeLa cells or Xenopus interphase egg extracts were analyzed by immunoprecipitation (IP) with either preimmune (P) or immune antibodies (I) against SA1 (444) or SA2 (446) and analyzed by SDS-PAGE and Western blotting (WB) with antibodies to the indicated proteins. (B) HeLa extracts were immunodepleted as in A and the resulting supernatant fractions were analyzed by SDS-PAGE and immunoblotting with SA1 (444) and SA2 (446) antibodies. Image published in: Sumara I et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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