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Figure 3. Purification of human cohesin complexes. (A) Immunoprecipitates (IP) obtained with either SA1/2 (447) antibodies or as a control with CDC27 antibodies (APC IP) from low-speed supernatant of extracts from logarithmically growing HeLa cells were analyzed by SDS-PAGE and silver staining. The positions of cohesin subunits, p85 and APC subunits as determined by immunoblotting are indicated. (B) Proteins were eluted with antigenic peptides from cohesin immunoprecipitates obtained as in A and further separated by sucrose density gradient centrifugation. Fractions 5–8 were analyzed by SDS-PAGE and immunoblotting with antibodies to the indicated proteins. (C) Sucrose density gradient fractions from the experiment shown in B were analyzed by SDS-PAGE and silver staining. Image published in: Sumara I et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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