Xenbase Image ID: 118758

Figure 7 Cyclin B proteolysis is not required for the mitotic dissociation of cohesins from chromatin. (A) High-speed supernatant (S100) fractions of Xenopus interphase extracts were incubated for 60 min at room temperature either with 3,200 Xenopus sperm nuclei/ul (left) or without nuclei (right). Nondegradable cyclin B Δ90 was then added to trigger entry into mitosis. At different time points either S100 samples (top) or chromatin reisolated from the reaction mixture by sucrose cushion centrifugation (bottom) were analyzed by SDS-PAGE and either PhosphorImaging (top) or immunoblotting with antibodies to the indicated proteins (bottom). The cell cycle state of the extracts was analyzed by monitoring the behavior of 35S-labeled CDC25 and cyclin B as in Fig. 5. TOPO II, topoisomerase II. Image published in: Sumara I et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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