XB-IMG-118798
Xenbase Image ID: 118798
Figure 7. Rescue of SSA by the xWRN protein. (A) Silver staining of the xWRN protein purified from Xenopus egg cytosol by conventional column chromatography (Yan et al., 1998). (B) Add-back of purified xWRN to the xWRN-depleted NPE. PRW4′ was incubated in xWRN-depleted NPE supplemented with xWRN (5 ng/μl final concentration) or buffer (ELB) for the indicated times and then analyzed by agarose gel electrophoresis. The SSA products include the band indicated by the arrow and a subset of the bands in the bracketed area. (C) Staining intensity plot of the lanes containing the 3-h repair products in (B). Image published in: Yan H et al. (2005) Copyright © 2005, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |