Xenbase Image ID: 118867

Figure 1. TACC3 forms a complex with XMAP215 in Xenopus egg extracts and in vitro. (A) Coimmunoprecipitation in Xenopus egg extracts. Immunoprecipitation (IP) was performed using control IgG (lane 1) or anti-TACC3 antibody (lane 2). The blots were probed with anti-TACC3 (top) or anti-XMAP215 (bottom). (B) Coimmunoprecipitation in the extracts of baculovirus-infected insect cells. Total lysates of TACC3 baculovirus single infected cells and TACC3 (lane 1) and XMAP215 virus double infected cells (lane 2) were prepared for immunoprecipitation. In each cell lysate, immunoprecipitation were performed using anti-TACC3 (lanes 3 and 4) or control IgG antibody (lanes 5 and 6). Total cell lysate and immunoprecipitates that were dissolved in sample buffer were subjected to SDS-PAGE, and the gel was stained with Coomassie brilliant blue. (C) Fractions from the top and bottom of a continuous sucrose density gradient centrifugation with purified proteins. Fractions collected after 3–15% continuous sucrose gradient centrifugation with TACC3 alone (top), XMAP215 alone (middle), or a mixture of TACC3 and XMAP215 (bottom) were subjected to SDS-PAGE. The gels were stained with Coomassie brilliant blue. (D) Gel filtration with purified proteins. TACC3 alone (top), XMAP215 alone (middle), or a mixture of TACC3 and XMAP215 (bottom) were analyzed by gel filtration. Fractions were collected and subjected to SDS-PAGE, and the gels were stained with Coomassie brilliant blue. Image published in: Kinoshita K et al. (2005) Copyright © 2005, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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