Xenbase Image ID: 118895

Figure 2. Xnf7 depletion accelerates exit from mitosis but does not cause spontaneous activation of the APC. (A–C) CSF-arrested Xenopus egg extracts were depleted, supplemented with demembranated sperm chromatin and CaCl2, and incubated at 23°C. (A and B) Aliquots removed at the indicated times after CaCl2 addition were analyzed by SDS-PAGE and immunoblotting with anti–cyclin B2 antibodies. (C) Aliquots of extract were removed at the indicated times and nuclear morphology was visualized by Hoechst 33258 staining and fluorescence microscopy. (D) CSF extracts were depleted, supplemented with demembranated sperm chromatin, and incubated at 23°C. Aliquots removed at the indicated times were analyzed as in A. (E) Interphase extracts were depleted, supplemented with 35S–cyclin B1, and incubated at 23°C. Aliquots removed at the indicated times were analyzed by SDS-PAGE and autoradiography. Image published in: Casaletto JB et al. (2005) Copyright © 2005, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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