Xenbase Image ID: 118935

Figure 6. Clustering of TirMC does not effectively trigger actin pedestal formation in cells that lack Nck. (A) TirMC was expressed in MEFs that express Nck1 (Nck +/− cells; top panels) and MEFs that do not express either Nck1 or Nck2 (Nck −/− cells; bottom panels). Cells were treated with anti-TirM antibodies and S. aureus particles and visualized as described in the Fig. 3 legend. (B) Nck-proficient MEFs (top panels) and Nck-deficient MEFs (bottom panels) were either mock transfected and infected with EPECΔtir + pTir (left panels) or were transfected with a plasmid encoding TirMC and challenged with E. coli expressing intimin (right panels). Cells were stained with antibodies to detect phosphotyrosine (green) and with DAPI to visualize bacterial DNA (blue). Image published in: Campellone KG et al. (2004) Copyright © 2004, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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