Xenbase Image ID: 118982

Figure 3. Differential binding activity of recombinant β-catenin as revealed by deletion analysis. (A) Schematic representation of β-catenin constructs. WT-myc-Xenopus β-catenin and GSK3β mutant (S/T>A residues 33, 37, 41, and 45) β-catenin were described previously by Guger and Gumbiner (2000). WT-human β-catenin-flag, ΔC695-flag and ΔN89-flag constructs were described in Kolligs et al. (1999). The myc-tagged, Xenopus β-catenin construct encoding only the arm repeat region of β-catenin was described previously (Funayama et al., 1995). (B) Recombinant β-catenin binding to cad-GST versus TCF-GST proteins. HEK293T cells were transfected with decreasing amounts of β-catenin plasmid and incubated in the presence (+) of Wnt3a conditioned media (CM). Cytosolic fractions were affinity precipitated and immunoblotted with anti-myc, -flag, or β-catenin antibodies. Input amounts of wild-type β-catenin, −ΔC695, and arm 12 constructs were the same in accordance with similar expression levels (not depicted). Image published in: Gottardi CJ and Gumbiner BM (2004) Copyright © 2004, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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