XB-IMG-119084
Xenbase Image ID: 119084
|
Figure 3. Overexpression of the PCM-1 deletion mutant 1–1468 delocalizes centrin, pericentrin, and endogenous PCM-1 to large cytoplasmic aggregates. (A and B) Control HeLa cells overexpressing β-galactosidase, stained in red; DNA stained in blue. (B) PCM-1 is stained in green. (C, E, G, and M) HeLa cells overexpressing PCM-1 deletion mutant 1–1468, stained in red; DNA stained in blue. The same cells were stained for (D) PCM-1, (F) centrin, (H) pericentrin, or (N) acetylated tubulin. The arrowhead in N indicates the position of the centriole pair. (I–L) CHO cells overexpressing PCM-1 mutant 1–1468 (I and K; red), stained for (J) γ-tubulin, or (L) ninein. Bars: (H and N) 10 μm; same magnifications in A, B, E–H and C, D, I–N, respectively. Image published in: Dammermann A and Merdes A (2002) Copyright © 2002, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |