XB-IMG-119088
Xenbase Image ID: 119088
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Figure 7. Microtubule anchoring to the centrosome depends on PCM-1. (A and B) Cos-7 cells expressing GFP-tagged PCM-1 deletion mutant 1–1468 (A, green). (A) Microtubules are stained in red, B shows microtubules only. (C and D) Control cells overexpressing GFP only (C, green). (C) Microtubules in red, (D) microtubules only. (E–J) Microtubule regrowth after nocodazole treatment of PtK2 cells. (E, G, and I) Staining of microtubules in red, GFP-tagged PCM-1 mutant 1–1468 in green; (F, H, and J) microtubules only. Time points at (E and F) 0 min, (G and H) 5 min, and (I and J) 60 min after removal of the drug. (K) Graph showing percentage of cells with radial microtubule organization at different time points after removal of nocodazole in untransfected cells (blue), cells overexpressing GFP (green), and cells overexpressing GFP-tagged PCM-1 mutant 1–1468 (red). Between 400 and 600 cells were counted for each time point. Bars: 10 μm (D and J). Image published in: Dammermann A and Merdes A (2002) Copyright © 2002, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |