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Figure 3. Cripto expression profile during the in vitro differentiation of ES cells. Total lysates of either undifferentiated ES cells or EBs at different days of differentiation (2–7 d), derived from either RI (wt) or DE7 (Cripto−/−) ES cells, were collected in lysis buffer and analyzed by Western blot using a polyclonal anti-Cripto antiserum (Minchiotti et al., 2000). Data were normalized to the expression level of Porin. Image published in: Parisi S et al. (2003) Copyright © 2003, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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