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Figure 8. Identification of Cripto key residues required for cardiac induction and differentiation. (A) Schematic representation of wt and mutant Cripto derivatives. (B) Western blot analysis of total lysates from 293EBNA cells transfected with either wt or mutant cripto derivatives. Jun-HA expression vector was cotransfected as an internal control. Either polyclonal anti-Cripto or monoclonal anti-HA antibodies were used to detect protein levels. (C) RNA expression levels of the cardiac αMHC and MLC2v genes during in vitro differentiation of Cripto−/− ES cells (days 5, 7, and 10) overexpressing either wt or mutant cripto derivatives. Expression level of HPRT gene was analyzed as an internal control. Image published in: Parisi S et al. (2003) Copyright © 2003, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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