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Figure 10. Exposure to Cerberus inhibits Cripto activity in cardiomyocyte differentiation assay. (A) Cerberus inhibits Cripto-dependent cardiomyocyte differentiation of Cripto−/− EBs. 2-d-old Cripto−/− EBs were cultured for 24 h in the presence of 100% (vol/vol) of media supernatant from 293T cells transiently expressing either Cerberus, Cerberus-S, or empty vector as control, in the presence of increasing amounts of recombinant Cripto protein. The number of Cripto−/− EBs containing beating areas was scored from day 8 to 12 of in vitro differentiation. Data are representative of two independent experiments. (B) Inhibition of Cripto by Cerberus-S is dose dependent. 2-d-old Cripto−/− EBs were cultured for 24 h with 10 μg/ml of soluble Cripto protein in the presence of increasing amounts (vol/vol) of media from 293T cells transiently expressing Cerberus-S. Cells were examined for cardiac differentiation as described above. Data are representative of two independent experiments. Image published in: Parisi S et al. (2003) Copyright © 2003, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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