Xenbase Image ID: 119267

Figure 5. TPX2 localizes Aurora-A to the spindle. (A) Western blot analysis of total extracts form HeLa cells treated for 36 h with a TPX2-specific siRNA duplex or a control duplex (GL2). Filters were probed for Aurora-A and α-tubulin as a loading control. (B and C) HeLa S3 cells were treated for 36 (A) or 48 h (B) with TPX2-specific siRNA duplex or a control duplex (GL2) and then fixed and stained with the antibodies indicated. Right panels show merged images, including DNA staining by DAPI. Bars, 10 μm. Image published in: Kufer TA et al. (2002) Copyright © 2002, The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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