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Figure 8. HSET antagonizes the plus end-directed activity of Eg5 during centrosome separation in vivo. Cultured CF-PAC1 cells were injected with antibodies specific for Eg5 (αEg5) or simultaneously with two antibodies, one specific for HSET and the other specific for Eg5 (αEg5/αHSET). Cells were monitored by phase-contrast microscopy until they entered mitosis, after which they were fixed and processed for indirect immunofluorescence using antibodies specific for γ-tubulin, the injected antibody/antibodies, and with the DNA-specific dye DAPI, as indicated. Arrows indicate separated centrosomes observed in double injected cells. Bar, 10 μm. Image published in: Mountain V et al. (1999) © 1999 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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