Xenbase Image ID: 119390

Figure 3. 6His-HsCen3p induces blastomeres undercleavage in Xenopus laevis embryos. A, Two-cell stage embryos microinjected either with 6 mg/ml His-HsCen3p (1), 12 mg/ml His-HsCen3p (2), 12 mg/ml heat-treated His-HsCen3p (3), or 12 mg/ml protease-treated His-HsCen3p (4) were observed 7 h after fertilization. Undercleaved blastomeres are present in the first three conditions (arrows). Bar, 1 mm. B, An embryo, injected with His-HsCen3p, methanol-fixed 8 h after microinjection, and labeled with anti–α-tubulin antibodies, contains large blastomeres with one or two microtubule asters in the injected half, whereas the uninjected cells gave rise to small blastomeres with one or two asters (inset). Bar, 50 μm. C, Large blastomere of a His-HsCen3p–injected embryo exhibiting one aster in a confocal section (1) and two additional asters in another section (2), whereas the uninjected cells gave rise to small blastomeres with one or two asters (bottom left). Bar, 50 μm. D, An embryo, injected with 12 mg/ml HsCen2p, methanol-fixed 8 h after microinjection, and labeled with anti–α-tubulin antibodies, contains large blastomeres with numerous asters (left) in the injected half, whereas the uninjected cells gave rise to small blastomeres with one or two asters (right). Bar, 50 μm. Image published in: Middendorp S et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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