XB-IMG-119393
Xenbase Image ID: 119393
|
Figure 6. Yeast cells expressing HsCen3p contain an unduplicated SPB. A and B, 12 h after induction, cells expressing HsCen3p were fixed and double labeled with an anti–α-tubulin (A1) or an anti-Cen3p (A2) antibody, or an anti-Spc110p (B1) or an anti-Cen3p (B2) antibody. DNA was labeled with DAPI (A3 and B3). Large-budded cells did not contain any spindle, but diverging microtubules radiating from the nucleus trapped in the bud neck (A1). Anti-Spc110p antibody detected only one dot on the nucleus trapped in the bud neck (B1). No specific localization of HsCen3p was detected (A2 and B2). Bar, 5 μm. C and C′, 12 h after induction, GFP-Spc42p bearing strain overexpressing HsCen3p was observed for GFP in the fluorescein channel (1), for DNA staining (DAPI; 2), and in Nomarski optics (3). In both cases, a single dot per nucleus was detected. Bar, 5 μm. D 1, Electron micrograph of a cell 12 h after induction of HsCen3p expression. A single SPB is observed at the bud neck. The contour of the nucleus can be easily identified by the nuclear envelope bearing nuclear pores (black dots). Microtubules diverging from the SPB can be seen in the nucleus and in the cytoplasm. D 2 and 3, SPBs of two individual cells. Note the bent profile of the unduplicated SPB. Bars, 0.3 μm. E, Five serial sections of a cell expressing HsCen3p 12 h after induction. A single unduplicated SPB is observed in sections 2, 3, and 4 (arrows) and is enlarged tenfold in the corresponding insets. Bars, 2 μm and 0.2 μm, respectively. Image published in: Middendorp S et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |