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Figure 7. Cdc31p can overcome HsCen3p-induced growth arrest in a dose-dependent manner. A, Cells expressing: a fusion between either amino acids 1–23 of HsCen3p and amino acids 18–161 of Cdc31p, or amino acids 1–17 of Cdc31p and amino acids 24–167 of HsCen3p; or overexpressing 2μ plasmids coding for HsCen3p and Cdc31p; or overexpressing both HsCen3p cloned on a centromeric (CEN) plasmid and Cdc31p cloned on a 2μ plasmid; or expressing HsCen3p and Cdc31-16p in a Δkar1 background were grown on glucose plates (repressive condition) or on raffinose + galactose plates (inducing condition) for 3 d at 30°C. For each condition, two dilutions were spotted. Cells expressing a fusion between amino acids 1–17 of Cdc31p and amino acids 24–167 of HsCen3p, both HsCen3p and Cdc31p, or expressing HsCen3p and Cdc31-16p in a Δkar1 background did not grow in inducing condition. B, 12 h after induction, GFP-Spc42p bearing strain overexpressing HsCen3p in a Δkar1 background was observed for GFP in the fluorescein channel, for DNA staining (DAPI), and in Nomarski optics. In both cases, a single dot per nucleus was detected. Bar, 5 μm. Image published in: Middendorp S et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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