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Figure 8. HsCen3p interacts with Kar1p extracts of yeast cells overexpressing HsCen3p, Cdc31p. HsCen3p in a Δkar1/cdc31-16 background, or HsCen2p were immunoprecipitated with anti-HsCen3p affinity-purified antibodies or with anti-HsCen2p antibodies. Immunoprecipitated proteins were separated on SDS-PAGE, transferred onto nitrocellulose, and revealed with anti-HsCen3p, anti-Cdc31p, or anti-Kar1p antibodies, or with anti-HsCen2p and anti-Kar1p antibodies. In cells overexpressing HsCen3p, HsCen3p and Kar1p are coimmunoprecipitated. It is noteworthy that in the conditions we have used, Cdc31p and Kar1p are not coimmunoprecipitated, whereas HsCen2p and Kar1p are. Image published in: Middendorp S et al. (2000) © 2000 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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