XB-IMG-119667
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Figure 2. Colocalization of FFA-1 and sites of DNA synthesis. (A–F) In normal reconstituted nuclei. Biotin-dCTP was added 5 min before fixation. (A–C) 45 min. (D–F) 60 min. (G–L) In reconstituted nuclei formed in the presence of aphidicolin. (G–I) An aphidicolin-arrested nucleus directly fixed and stained. Biotin-dCTP was added to the reaction at the beginning. (H–L) An aphidicolin-arrested nucleus pelleted through 1 M sucrose cushion and then labeled with dATP, dGTP, TTP, and biotin-dCTP. (A, D, G, and J) FFA-1 staining. (B, E, H, and K) Biotin staining. (C, F, I, and L) Merge of FFA-1 staining and biotin staining. Image published in: Chen CY et al. (2001) © 2001 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |