Xenbase Image ID: 119670

Figure 5. Formation of hybrid foci. (A–F) Nuclei were reconstituted around sperm chromatin for 52 min in the presence of GST-Xho and stained with the affinity-purified anti-GST (A and D), RPA (B), or FFA-1 (E). (C) Merge of GST staining and RPA staining. (F) Merge of GST staining and FFA-1 staining. The anti–FFA-1 antibody is against the COOH-terminal end of FFA-1 and does not react with GST-Xho. (G–O) Nuclei reconstituted in the presence of GST-Stu/Xho (G–I), GST-Stu (J–L), and GST (M–O) for 52 min. (G, J, and M) GST staining (green). (H, K, and N) RPA staining (red). (I, L, and O) Merge of GST and RPA staining. Image published in: Chen CY et al. (2001) © 2001 The Rockefeller University Press. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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